This collection consists of:

Chase's correspondence with Mildred Gilman Wohlforth (1896–1994), the Library of Congress Copyright Office, C.T. Millspaugh (1854–1923), James Francis McBride (1892–1976), Walter V. Brown (1913–1977), Erik Ekman (1883–1931), and Ezra Taft Benson (1899–1994), along with some letters written by A.S. Hitchcock (1865–1935) to Ekman.

Correspondence about Chase from Wohlforth with Florence van Esteline (?), John Reeder (1914–2009), Charlotte Goodding Reeder (1916–2009), Virginius Chase (1876–1966), and Grace (?).

Copies of academic articles by Chase that had been published for scientific journals and the Field Museum of Natural History in Chicago, Illinois.

Various newspaper articles pertaining to Agnes and Virginius Chase.

Various certificates rewarded to Chase.

Copies of a business license and death certificates pertaining to Chase and her family.

A scrapbook with writings, illustrations, and photographs related to Chase's pet squirrel, Toodles, dated December 1914.

Chase's honorary doctorate received in 1958 from the University of Illinois along with the hood and gown she wore at the graduation ceremony.

The João VI medal and honorary diploma awarded to Chase by the Rio de Janeiro Botanical Garden in 1958.

A 1962 draft of the preface to Index to Grass Species by Jason R. Swallen (1903–1991).

An undated, handmade booklet with pressed specimens of various flowers, titled "Western Wildflowers, Gathered by Mary Agnes Chase."

Copies of undated botanical illustrations of grasses.

Undated notes by Wohlforth with biographical, genealogical, and professional details concerning Chase.

Two undated drafts of Wohlforth's biographical essay on Chase, "Agnes Chase: My Most Unforgettable Character", with handwritten notes.